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Toxicity Research:

The following tests were conducted by SGS U.S. Testing Company. The SGS network comprises more than 1,000 offices and laboratories and 37,000 employees, in well over 120 countries. It is one of the most respected testing labs in the world.

Creative Labs, Inc. of Boca Raton, Florida, conducted a series of thorough toxicity tests on Area International’s H2O-BOO2ST® OXYGEN. The purpose of the tests was to determine if H2O-BOO2ST® OXYGEN had any ocular, dermal, inhalation or internal toxicity. To determine toxicity, safety tests were conducted on lab animals. The majority of test animals were rodents (primarily mice and rats). SGS tested H2O-BOO2ST® OXYGEN on Hartley Strain Albino Guinea Pigs, New Zealand Albino Rabbits and Sprague-Dawley Rats,

It is important to note that scientists have dramatically reduced the number of animals used in product safety testing. Animal rights activists have led the public to believe that the use of laboratory animals can be eliminated in this field altogether. It is often claimed that valid "alternatives" to the use of animal tests already exist to evaluate product safety. This is simply not true. In fact, companies that claim their products are not animal tested and, therefore, are "cruelty-free", mislead consumers, since almost all products, or the chemical compounds that comprise them, were at one time previously tested on animals.

While some “alternatives” to the use of animals have proven to be of value, the fact remains that most “alternatives” can only be used to screen for only a few toxic effects. In the final analysis, some animal models must be used. Not using animal tests would subject humans to unreasonable risks.

We believe that, as a manufacturer and distributor of nutritional and skin care products, we have both an ethical and a legal obligation to protect the safety of consumers. The purpose of safety (toxicity) testing is to ensure that a product is safe when used as directed. The results of these tests also provide scientific data for poison control centers and emergency room personnel should a product be misused.

“Acute Toxicity” is the ability of a substance to cause severe biological harm or death soon after a single exposure or dose. It also refers to any poisonous effect resulting from a single short-term exposure to a toxic substance. A poison, of course, is any substance that adversely affects someone's health by causing injury, illness, or death.

SGS conducted six different toxicity tests on H2O-BOO2ST® OXYGEN. For purposes of these studies, the “lethal dose” (LD) or “lethal concentration” (LC) needed to be quantified. Once this amount had been determined, the amount was divided in half and was represented as half (50%) of the amount of a substance that can cause death when consumed or used. This is what is meant by the terms LD50 and LC50.

Of special note, in reviewing the tests conducted by SGS, is the fact that there were no reported incidences of death, (see necropsy findings,) for any of the laboratory animals. In fact, without exception, all of the laboratory animals exhibited healthy and weight gains indicating that H2O-BOO2ST® OXYGEN did not have any adverse affect on normal metabolic functions.



The tests conducted by SGS were:

Acute Oral Toxicity Testing: This test determines whether a substance is toxic when consumed. There are three degrees defining oral toxicity: (1) Non-Toxic: The probable lethal dose of undiluted substance to 50% of the test animals determined from ingestion studies (LD50) is greater than 5 grams per kilogram of body weight; (2) Toxic: The probably lethal dose of undiluted substance to 50% of the test animals determined from ingestion studies (LD50) is greater than 50 milligrams and less than or equal to 5 grams per kilogram of body weight; (3) Highly Toxic: The probably lethal dose of undiluted substance to 50% of the test animals determined from ingestion studies (LD50) is less than or equal to 50 milligrams per kilogram of body weight.

Acute Dermal Toxicity Test (LD50): This test determines whether a substance posses the ability to become toxic to the skin. There are three different degrees defining acute dermal toxicity: (1) Non-Toxic: The lethal dose of the undiluted substance to 50% of the test animals determined from dermal toxicity studies (LD50) is greater than 2 grams per kilogram of body weight; (2) Toxic: The lethal dose of the undiluted substance to 50% of the test animals determined from dermal toxicity studies (LD50) is greater than 200 milligrams and less than or equal to 2 grams per kilogram of body weight: (3) Highly Toxic: The lethal dose of the undiluted substance to 50% of the test animals determined from dermal toxicity studies (LD50) is less than or equal to 200 milligrams per kilogram of body weight.

Acute Dermal Irritation/Corrosion Test: This test determines whether a substance can irritate or can be corrosive to the skin. There are four different degrees defining acute dermal irritation: (1) Practically Non-Irritating: The undiluted substance causes no noticeable irritation, or causes slight inflammation; (2) Moderately Irritating: The undiluted substance causes well-defined inflammation during the study period; (3) Primary Skin Irritant: The undiluted substance causes moderate to severe inflammation during the study period; (4) Corrosive: The undiluted substance causes visible destruction or irreversible alterations of the tissue structure at the site of contact during the study period.

Acute Inhalation (LC50) Toxicity Testing: This test determines whether a substance can be toxic when inhaled. There are three degrees defining acute inhalation toxicity: (1) Non-Toxic: The lethal concentration of the undiluted substance to 50% of the test animals (LC50) is greater than 200 milligrams per liter by volume when inhaled continuously for one hour or less; (2) Toxic: The lethal concentration of the undiluted substance to 50% of the test animals (LC50) is greater than 2 milligrams and less than or equal to 200 milligrams per liter by volume when inhaled continuously for one hour or less; (3) Highly Toxic: The lethal concentration of the undiluted product to 50% of the test animals (LC50) is less than or equal to 2 milligrams per liter by volume when inhaled continuously for one hour or less.

Skin Sensitization Test: This test determines whether a substance possesses a delayed-contact dermal sensitization response. There are four degrees defining skin sensitization: (1) Practically Non-Irritating: The undiluted substance causes no noticeable irritation, or causes slight inflammation; (2) Moderately Irritating: The undiluted substance causes well-defined inflammation during the study period; (3) Primary Skin Irritant: The undiluted substance causes moderate to severe inflammation during the study period; (4) Corrosive: The undiluted substance causes visible destruction or irreversible alterations of the tissue structure at the site of contact on the skin during the study period.

Eye Irritation Test: This test determines whether a substance irritates the eye. There are three degrees defining eye irritation: (1) Practically Non-Irritating: The undiluted substance, when instilled into the eyes produces no noticeable irritation, or slight transient conjunctival irritation; (2) Slightly Irritating: The undiluted substance, when instilled into the eyes, produces slight to moderate conjunctival irritation, slight corneal involvement, and/or slight iritis (the effects clear within 7 days); (3) Moderately Irritating: The undiluted substance, when instilled into the eyes, produces moderate corneal involvement with or without severe iritis. The effects clear within 21 days.


Here is a summary of SGS’s findings:

Acute Oral Toxicity Testing: “When tested as specified, the submitted test substance, H2O-BOO2ST® OXYGEN at Full Strength Concentrated Solution, was determined to have an acute oral LD50 greater than 5.0 g/kg.” (Non-Toxic)

Acute Dermal Toxicity Test (LD50): “When tested as specified, H2O-BOO2ST® OXYGEN at 20% Strength Concentrated Solution was not acutely toxic to the test animals following dermal application of the test substance at 2.0 g/kg.” (Non-Toxic)

Acute Dermal Irritation/Corrosion Test: “When tested as specified, H2O-BOO2ST® OXYGEN at 20% Strength Concentrated Solution, did not induce dermal irritation in the six test animals following a 4-hour dermal exposure.” (Practically Non-Irritating)

Acute Inhalation (LC50) Toxicity Testing: “When tested as specified, H2O-BOO2ST® OXYGEN at 40% Strength Concentrated Solution, was not acutely toxic to the test animals following a four hour inhalation exposure at a normal concentration of 23.9 mg/L (actual concentration was 14.6 mg/L). The LC50 was estimated to be greater than 23.9 mg/L (actual concentration was 14.6 mg/L).”

Skin Sensitization Test: “When tested as specified, H2O-BOO2ST® OXYGEN at 20% Strength Concentrated Solution, was not considered to be a skin sensitizing agent. There were no significant differences in skin irritations observed in the test animals and in the control animals on challenge.”

Eye Irritation Test: “When tested as specified, the submitted test substance, H2O-BOO2ST® OXYGEN at 20% Strength Concentrated Solution, was not an eye irritant.”


Conclusion:
In conclusion, H2O-BOO2ST® was not found to be either toxic or an irritant to the skin, eyes or the respiratory system. Neither was the solution found to be toxic when consumed.

 

Additional Research On H2O-BOO2ST® OXYGEN

The Affect of H2O-BOO2ST® on the Partial Pressure of Oxygen in Arterial Blood
Research Reference: To determine whether the partial pressure of oxygen in arterial blood (PaO2) in the forearm after rest would change following the oral consumption of H2O-BOO2ST® formulation. Three healthy males were tested before and after consuming the solution over a period of 240 minutes. The dosage was 6 ml of H2O-BOO2ST® per person.

"Each subject's partial pressure of oxygen was relatively stable prior to consumption, but then rose immediately after consumption. The partial pressure of oxygen peaked 90 to 120 minutes after consumption, after which it gradually dropped, reaching its pre-consumption level. In a subject with a particularly low baseline, a significant increase was observed." (Suntory study: May 1996)

 

H2O-BOO2ST® contains the most concentrated source of pH balanced bio-available oxygen of any supplement available today.
Research Reference: Dissolved oxygen tests were conducted on H2O-BOO2ST® and diluted H2O-BOO2ST® by various labs throughout the U.S. In every instance, the dissolved oxygen levels of H2O-BOO2ST® were significantly higher than dissolved oxygen levels of tap or bottled water. In most instances the levels of dissolved oxygen present were thousands of times higher. “...the actual D.O. (dissolved oxygen) mathematical extrapolation would be 92,000 p.p.m. (parts per million of oxygen).” Dr. J. Michael Sadowski, Ph.D., Essential Green, Inc.

 

H2O-BOO2ST® has been tested on U.S. Marines in a double blind study.
Research Reference: In a controlled double blind study conducted by the U.S. Naval Warfare Training Center (San Diego, CA) and the University of Utah (Nutrition Department) it was found that H2O-BOO2ST® group experienced greater stamina and endurance, reduced muscle fatigue, more energy, a greater feeling of strength and better performance than did the placebo group.

 

H2O-BOO2ST® has proven anti-microbial disinfectant properties.
Research Reference: Minimum Inhibitory Concentration Anti-microbial Activity tests were conducted by a major U.S. testing facility. It was found that H2O-BOO2ST® diluted to 40% strength conclusively inhibited the growth of the four basic test organisms accepted by the FDA and EPA as the standard organisms used to qualify a product as an antimicrobial disinfectant. One of the test organisms killed by H2O-BOO2ST®, E. coli 0157, was reportedly responsible for serious illnesses and deaths in Japan.

H2O-BOO2ST® was tested for its minimum inhibitory concentration (MIC) against four standard test organisms: Escheria coli O157:H7, Pseudomonas aeruginosa, Salmonella choleraesuis and Staphyloccus aureus. H2O-BOO2ST® clearly inhibited the growth of all four test organisms. (SGS U.S. Testing Company, Inc.)

 
Here’s What Researchers Have Said About H2O-BOO2ST®
 
H2O-BOO2ST® has been independently tested at a number of Universities, by respected scientists and at highly regarded laboratories in the world. No other oxygen supplement has undergone as much scrutiny. Here is what the professionals have written about H2O-BOO2ST®:

H2O-BOO2ST® is undoubtedly a new powerful tool in the modern world of medicine. The more we get to know about it, the more we can treat without side effects. H2O-BOO2ST® is a tool, a key to a noble dream for a better world, better life quality and life itself. H2O-BOO2ST® works as a strong antiseptic on the wound, killing anaerobic and fungal microorganisms. H2O-BOO2ST® increases the healing process. H2O-BOO2ST® was effective in dilutions and in the oral treatment of Stomatitis Aphthosa in the mouth and gums. H2O-BOO2ST® was effective as a topical treatment against Oral Cardidiasis and tongue’s fungal plaques. H2O-BOO2ST® was effective on degenerative lip dermatitis and Angulus Infectiosus in mouth angles. H2O-BOO2ST® was effective on eczematic areas as a moisturizing and skin repair agent.
Dr. Marios L. Christofinis, M.D., Ph.D.

 

I believe H2O-BOO2ST®, because of its inherent “energy factor” potential may just be the “Rosetta Stone” that unblocks the pathogenetic mechanisms of disease in general and provides the knowledge for proper disease prevention management. H2O-BOO2ST® is not simply an important nutrient supplement but a dynamic energy molecule. H2O-BOO2ST® has an extremely high ORP (Oxidation Reduction Potential) of 950 mV. That means H2O-BOO2ST® possesses energy that can be transferred to the surrounding environment, potentiating the bioenergetic processes and correcting or reversing underlying cellular dysfunctions. In other words, H2O-BOO2ST® works like a bright new cellular battery.
Menicos A. Spartalis, M.D., Vascular Surgeon

 

In the beginning I used H2O-BOO2ST® rather cautiously for cleaning the face for local uses thus assessing the action of this product as a bactericide. Later I started recommending it for the detoxification of the organism thus testing its activity for neutralizing free radicals. I gave it to my patients with tired and rather lifeless skin, with black circles under the eyes or individuals suffering from eczema and dermatitis. The results were fantastic and this made me bolder and able to recommend it to athletes, smokers, persons with feebleness, lack of energy, chronic bronchitis, asthma and to people with frequent viral infections due to a weakness in their immune system as well as to patients with bed-sores, etc. In all these cases the results have been excellent.
Dr. Margarita Chilindri, M.D., Pathology and Dermatology

 

From the results it is concluded that the ingestion of the H2O-BOO2ST® considerably affects the tolerance levels of lactate acid in the blood and improves VO2max.
Nicos Yiannaki Pericleous, M.Sc., ACSM

 

The potential role of H2O-BOO2ST® as an ergogenic aid in sports performance enhancement is still to be fully understood. Available scientific data and information on this subject presents a whole range of possibilities fur further studies. In this study it was established that there was not a far-reaching effect of H2O-BOO2ST® on the same subjects in terms of performance enhancement during trial test sprints. However, a definite improvement was noticed in between the sprints of the trials with placebo and the controlled H2O-BOO2ST®. This would indicate the fact that athletes who consumed H2O-BOO2ST® were able to reproduce similar and sustained effort during both sprints as compared to those that consumed the placebo.
Dr. Hj Danish Zaheer Hj Zaheeruddin MD., PhD. and David Hennessy Bsc (Hons), Sports Medicine & Research Centre, Department of Youth & Sports, Brunei Darussalam

 

From my clinical experience this product can be used in all aspects of the oral medicine and surgery, for example
◊ As an irrigant for the cleaning and prophylaxis of the oral cavity
◊ For the treatment of periodontitis and gingivitis
◊ Post surgical procedures and post extracting treatment
◊ For biological treatment and elimination of amalgam filling and heavy metal drainage
◊ For therapy in a systemic disease, and many other applications
In conclusion, I would like to say that H2O-BOO2ST®, because of its bio-friendly capabilities, it has the potential to be a powerful source for clinical or paramedical practice.
Dr. Stylianou Panayiotis, M.D.

 

In short, in both measurable parameters and subjective observations, the test subjects in the group treated with the H2O-BOO2ST® experienced the following to a greater degree than the control group: Greater stamina and endurance, Reduced muscle fatigue, More energy, Less “out of breath”, Greater feeling of strength, Felt that the product helped them perform better.
“Effect of stabilized oxygen consumed with water on blood and urine markers of oxidative stress and blood oxygen saturation during extended military mountaineering training at moderate altitude.” Eldon W. Askew, Ph.D. Department Chair, School of Nutrition, University of Utah, Donald E. Roberts, Ph.D., James E. Reading, M.A., Jeffrey M. Pfeiffer, M.S., Lt. Lance Orr, MC, USNR.

 

Blood gas analyses conducted on the participants' arterial blood samples showed definite increases in arterial blood oxygen (PaO2), as well as elevated discharges of carbon dioxide waste matter. Older subjects appeared to respond better to the test product's therapeutic actions, than was reported from younger recipients. The inclusion of an iron supplement with the test product indicates a helpful role in how the body utilizes "Vitamin O". However, non-iron subjects also receiving test product posted higher-than-expected hemoglobin values, which suggests that the apparent blood-building action of "Vitamin O" can happen also without iron-dependency. A general stabilization of arterial blood oxygen levels following three months of steady supplementation with the test product became evident, but could change if daily intake were temporarily discontinued.
Dr. John Heinerman, Ph.D


 

Purpose of H2O-BOO2ST® Oxygen:
H2O-BOO2ST® OXYGEN is designed to be taken orally as an additional source of minerals and bio-available oxygen molecules which may be absorbed into the body through the digestive tract into the blood plasma. There the bio-available oxygen may be available to the red blood cells (hemoglobin) and may increase the amount of available dissolved oxygen in the blood plasma which may be used by the cells for normal cellular metabolism.

H2O-BOO2ST® OXYGEN may also be used effectively as a natural biocidal topical disinfectant though it has not been approved for such uses by the U.S. F.D.A. or U.S. E.P.A.

H2O-BOO2ST® OXYGEN is manufactured and distributed as a vitamin supplement and not as a prescription nor as a medical drug. The sole active ingredient in H2O-BOO2ST® OXYGEN is dissolved oxygen molecules in a saline (NaCl) water medium which also includes a variety of trace and essential minerals.

H2O-BOO2ST® INGREDIENTS
Contains the following ingredients (by percentage of total volume):
Distilled Water (72.72%) CAS 7732-18.5
Molecules of H2O-BOO2ST® OXYGEN
(25.0%)
Dissolved Trace Minerals and Sodium Chloride (2.28%) CAS 7647-14-5


Other Analytical Specifications:
Specific Gravity: 1.016 23º/23º C

Density:
1.016 g/cm3
pH:
8.46
Shelf Life/Stability:
Three (3) Years from Manufacturing Date

Non-Toxicity:
H2O-BOO2ST® OXYGEN is completely non-toxic, biodegradable, non-caustic and non-flammable. H2O-BOO2ST® OXYGEN
contains natural ingredients including iodized sea salt and distilled water. It contains no microbiological contaminants, no pesticides, is naturally biocidal and contains no animal nor vegetable products or by-products.

 

AGRICULTURAL

H2O-BOO2ST® has potential applications for usage in the agricultural industry.
Research Reference: In a controlled study conducted at California Polytechnic State University at San Luis Obispo (Biological Sciences Department), it was determined that H2O-BOO2ST® effectively prevents the germination or growth of bacteria and fungal plant pathogens.

“Preliminary in vitro tests showed H2O-BOO2ST® to effectively prevent the germination or growth of bacteria and fungal plant pathogens.” Dr. M. Yoshimura, Ph.D., Phytopathology Department

“...the 50% concentration of H2O-BOO2ST® inhibits spore germination in Alternaria zinniae, a result found previously.” J. Orlando, Biological Sciences Department, Cal Poly State University

 

H2O-BOO2ST® has been used effectively as a preservative for cut flowers significantly extending the shelf life in one study.
Research Reference: In a controlled study at the Environmental Horticulture Science Department at California Polytechnic State University, San Luis Obispo, it was concluded that:

“In this comparison study the results appear probable that the application of H2O-BOO2ST® to the vase water of freshly cut carnation flowers lengthens the vase life. The results are worthy of further study. The results of the present study show potential towards its future use in this area of vase life longevity.” Bliss Quimby: Effect of H2O-BOO2ST® in Comparison to Floralife® and Deionized Water on the Prolongation of the Vase Life of Cut Carnation Flowers

 

H2O-BOO2ST® may be effective in preventing frost damage to produce crops, may increase seed germination rates and reduce microorganism’s growth in potting soil.
Research Reference: In a limited study at the Agronomy and Soils Department at the University of Minnesota, it was determined that applications of H2O-BOO2ST® on celery and parsley seeds resulted in from an 8 % to 14% increase in germination rates. In another study at the same university it was determined that: “...stabilized oxygen makes sugar beet seedlings stronger so that they are better able to resist frost.” Dr. Charles Habstritt, Ph.D., Professor of Agronomy and Soils.

 

 


 

NOTICE: This information is intended for educational purposes only and should not be construed as medical advice. These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure, or prevent any disease. Consult a qualified medical practitioner before taking any dietary supplements.

This information may not be reproduced or utilized in any form or by any means, in whole or in part, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without prior written permission. 

 

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